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Optisk Clearing af Mouse centralnervesystemet Brug Passiv CLARITY
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Neurociência
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JoVE Journal Neurociência
Optical Clearing of the Mouse Central Nervous System Using Passive CLARITY

Optisk Clearing af Mouse centralnervesystemet Brug Passiv CLARITY

DOI:
10.3791/54025-v

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10:28 min

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June 30, 2016

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, , ,
1Department of Neurology, David Geffen School of Medicine,University of California, Los Angeles, 2W.M. Keck Science Department,Claremont McKenna, Pitzer & Scripps Colleges

Capítulos

  • 00:05Título
  • 00:48Hydrogel Infiltration
  • 01:32Polymerization
  • 03:03Lipid Removal
  • 03:57Molecular Staining
  • 04:32Refractive Index Matching
  • 06:36Imaging the Cleared Sample
  • 08:43Results: THY1-YFP Expression in the Brain and Spinal Cord
  • 09:44Conclusion

Summary

Tadução automática

Tadução automática

Optical clearing techniques are revolutionizing the way tissues are visualized. In this report we describe modifications of the original Clear Lipid-exchanged Acrylamide-hybridized Rigid Imaging-compatible Tissue-hYdrogel (CLARITY) protocol that yields more consistent and less expensive results.

Tags

Keywords: Optical ClearingCLARITYMouse Central Nervous SystemHydrogelFluorescent ProteinsDeoxygenationPolymerizationLipid Removal

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