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돼지 피하 지방이 조직에서 분리 및 지방이 유래 줄기 세포의 분화
JoVE Journal
Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Isolation and Differentiation of Adipose-Derived Stem Cells from Porcine Subcutaneous Adipose Tissues
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돼지 피하 지방이 조직에서 분리 및 지방이 유래 줄기 세포의 분화

28,656 Views

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09:20 min

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March 31, 2016

DOI:

10.3791/53886-v

DOI:
10.3791/53886-v

•

09:20 min
March 31, 2016

•

13 Views
, , , , , ,
1Institute of Biotechnology,National Taiwan University, 2Department of Animal Science and Technology,National Taiwan University, 3Department of Surgery,National Taiwan University Hospital and College of Medicine

Summary

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This protocol describes the isolation of pig adipose-derived stem cells (pADSC) from subcutaneous adipose tissues with examination of multipotency. The multipotent pADSC are used to delineate processes of adipocyte differentiation and study transdifferentiation into multiple cell lineages of mesodermal mesenchyme or further lineages of ectoderm and endoderm for regenerative studies.

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Chen, Y., Liu, H., Chang, Y., Cheng, Y., Mersmann, H. J., Kuo, W., Ding, S. Isolation and Differentiation of Adipose-Derived Stem Cells from Porcine Subcutaneous Adipose Tissues. J. Vis. Exp. (109), e53886, doi:10.3791/53886 (2016).

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