Journal
/
Medicina
/
TRUE genet Slå: Screening av en Heptamer-type liten guide RNA bibliotek for potensielle kreft Terapeutiske midler
JoVE Journal
Medicina
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Medicina
TRUE Gene Silencing: Screening of a Heptamer-type Small Guide RNA Library for Potential Cancer Therapeutic Agents

TRUE genet Slå: Screening av en Heptamer-type liten guide RNA bibliotek for potensielle kreft Terapeutiske midler

DOI:
10.3791/53879-v

•

09:18 min

•

June 02, 2016

•

, , ,
1Research Institute for Healthy Living,Niigata University of Pharmacy and Applied Life Sciences

Capítulos

  • 00:05Título
  • 00:41Construction of a Heptamer-type sgRNA Library
  • 04:26Screening of the sgRNA Library for Cell Viability
  • 05:44Evaluation of Effective sgRNAs by Flow Cytometry
  • 07:15Effects of Heptamer-type sgRNAs on Leukemia and Myeloma Cell Lines
  • 08:41Conclusion

Summary

Tadução automática

Tadução automática

Her presenterer vi en protokoll for screening av en heptamer-type sgRNA bibliotek for potensielle terapeutiske legemidler mot blodkreft.

Tags

Keywords: TRUE Gene SilencingSmall Guide RNA (sgRNA)Heptamer-type SgRNA LibraryTRNase ZLRNA-directed Gene SilencingCancer Therapeutic AgentsBlood CancersMyelomaLeukemiaCell ViabilityApoptosisFlow Cytometry

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

check_url/pt/53879?article_type=v

Bronchial Thermoplasty: A Novel Therapeutic Approach to Severe Asthma
check_url/pt/53879?article_type=v

Orthotopic Xenografting of Human Luciferase-Tagged Malignant Peripheral Nerve Sheath Tumor Cells for in vivo Testing of Candidate Therapeutic Agents
check_url/pt/53879?article_type=v

Delivery of Therapeutic Agents Through Intracerebroventricular (ICV) and Intravenous (IV) Injection in Mice
check_url/pt/53879?article_type=v

A Simple Guide Screw Method for Intracranial Xenograft Studies in Mice
check_url/pt/53879?article_type=v

DNA Vector-baserte RNA interferens for å studere gen-funksjon hos Cancer
check_url/pt/53879?article_type=v

A Novel Surgical Approach for Intratracheal Administration of Bioactive Agents in a Fetal Mouse Model
check_url/pt/53879?article_type=v

Screening for Melanom Modifiers ved hjelp av en Sebrafisk stedegen Tumor Model
check_url/pt/53879?article_type=v

En orthotopic Blærekreft Modell for Gene Levering Studies
check_url/pt/53879?article_type=v

En Multiplexed Luciferase-basert screening plattform for avhør kreft-assosiert Signal Transduksjon i dyrkede celler
check_url/pt/53879?article_type=v

Fokus Dannelse: En Cell-basert analyse for å bestemme onkogent potensiale av en Gene

Read Article