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Scrutando Cervello polisomi con microscopia a forza atomica
JoVE Journal
Neurociência
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JoVE Journal Neurociência
Peering at Brain Polysomes with Atomic Force Microscopy
DOI:

08:49 min

March 16, 2016

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Capítulos

  • 00:05Título
  • 01:34Preparation of Polysome Lysate and Sucrose Gradient Fractionation
  • 03:40Atomic Force Microscopy Sample Preparation
  • 05:24AFM Imaging
  • 06:40Results: AFM Reveals Ribosome/Polysome Details
  • 07:39Conclusion

Summary

Tadução automática

While ribosome structure has been extensively characterized, the organization of polysomes is still understudied. To overcome this lack of knowledge, we present here a detailed preparation protocol for accurate imaging of mammalian polysomes by atomic force microscopy (AFM) in air and liquid.

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