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정성 및 정량 검출을위한 시험 법 및 단백질 항생제 특성
JoVE Journal
Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Qualitative and Quantitative Assays for Detection and Characterization of Protein Antimicrobials
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정성 및 정량 검출을위한 시험 법 및 단백질 항생제 특성

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10:50 min

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April 10, 2016

DOI:

10.3791/53819-v

DOI:
10.3791/53819-v

•

10:50 min
April 10, 2016

•

10 Views
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1Department of Advanced Technology,The MITRE Corporation, 2Center for Biomedical Engineering,Brown University, 3The MITRE Corporation

Summary

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Here, we present protocols to rapidly screen and characterize enzymes for antimicrobial activity. The microslide diffusion assay and the dye-release assay utilize target bacterial substrates for qualitative and quantitative enzymatic activity evaluation.

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Protein AntimicrobialsAntimicrobial ActivityMicroslide Diffusion AssayDye-release AssayBCA Protein AssayAgarose SolutionSodium AzideBacterial SubstrateMcFarland Turbidity StandardSerial DilutionBovine Serum AlbuminPositive Control

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Farris, M. H., Ford, K. A., Doyle, R. C. Qualitative and Quantitative Assays for Detection and Characterization of Protein Antimicrobials. J. Vis. Exp. (110), e53819, doi:10.3791/53819 (2016).

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