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軸索分岐と神経形態形成中に形質膜配信の役割を定義するために組み合わせる方法論を活用
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Neurociência
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JoVE Journal Neurociência
Utilizing Combined Methodologies to Define the Role of Plasma Membrane Delivery During Axon Branching and Neuronal Morphogenesis
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軸索分岐と神経形態形成中に形質膜配信の役割を定義するために組み合わせる方法論を活用

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14:28 min

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March 16, 2016

DOI:

10.3791/53743-v

DOI:
10.3791/53743-v

•

14:28 min
March 16, 2016

•

5 Views
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1Curriculum in Neurobiology,The University of North Carolina at Chapel Hill, 2Department of Cell Biology and Physiology,The University of North Carolina at Chapel Hill

Summary

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Light microscopy techniques coupled with biochemical assays elucidate the involvement of SNARE-mediated exocytosis in netrin-dependent axon branching. This combination of techniques permits identification of molecular mechanisms controlling axon branching and cell shape change.

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Plasma MembraneAxon BranchingNeuronal MorphogenesisLive Cell ImagingBiochemical ApproachesCortical NeuronsNetrin-1HomogenizationTriton X-100Bradford AssaySDS-PAGEWestern BlottingTIRF Microscopy

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Winkle, C. C., Hanlin, C. C., Gupton, S. L. Utilizing Combined Methodologies to Define the Role of Plasma Membrane Delivery During Axon Branching and Neuronal Morphogenesis. J. Vis. Exp. (109), e53743, doi:10.3791/53743 (2016).

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