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Biologia do Desenvolvimento
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Isolation and Characterization of Mouse Antral Oocytes Based on Nucleolar Chromatin Organization
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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Isolation and Characterization of Mouse Antral Oocytes Based on Nucleolar Chromatin Organization

Isolation and Characterization of Mouse Antral Oocytes Based on Nucleolar Chromatin Organization

DOI:
10.3791/53616-v

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07:16 min

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January 07, 2016

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1Research Center for Regenerative Medicine,Fondazione IRCCS Policlinico San Matteo, 2Department of Biology and Biotechnology “L. Spallanzani”,University of Pavia

Capítulos

  • 00:05Título
  • 00:47Ovary Harvest
  • 02:29Antral Oocyte Isolation
  • 04:12Antral Oocyte Sorting
  • 05:28Results: Representative Oocytes Stained and Sorted with Hoechst33342
  • 05:53Conclusion

Summary

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Here we present a protocol for the characterization of mouse antral oocytes based on nucleolar organization.

Tags

IsolationCharacterizationMouse Antral OocytesNucleolar Chromatin OrganizationEmbryonic DevelopmentBiologia do DesenvolvimentoDistinguishIsolateSN (surrounded Nucleolus)NSN (non-surrounded Nucleolus)Mammalian OvaryHarvest OvariesPregnant Mare Serum GonadotropinM2 MediumMineral OilPetri DishIncubator37 Degrees CelsiusCarbon Dioxide IncubatorAbdominal Wall IncisionPeritoneum IncisionUterine Tubes

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