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Generating CRISPR/Cas9 Mediated Monoallelic Deletions to Study Enhancer Function in Mouse Embryonic Stem Cells
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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Generating CRISPR/Cas9 Mediated Monoallelic Deletions to Study Enhancer Function in Mouse Embryonic Stem Cells

Generating CRISPR/Cas9 Mediated Monoallelic Deletions to Study Enhancer Function in Mouse Embryonic Stem Cells

13,810 Views

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11:31 min

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April 02, 2016

DOI:

10.3791/53552-v

DOI:
10.3791/53552-v

•

11:31 min
April 02, 2016

•

3 Views
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1Department of Cell and Systems Biology,University of Toronto

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CRISPR/Cas9Monoallelic DeletionEnhancer FunctionMouse Embryonic Stem CellsAllele-specificFunctional GenomicsStem Cell FateTransfectionElectroporationFlow Cytometry

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Moorthy, S. D., Mitchell, J. A. Generating CRISPR/Cas9 Mediated Monoallelic Deletions to Study Enhancer Function in Mouse Embryonic Stem Cells. J. Vis. Exp. (110), e53552, doi:10.3791/53552 (2016).

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