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İnsan Periferik Kan immünfenotipik Analizi Antikor Kokteyller Hazırlama A Yarı-otomatik Yaklaşım
JoVE Journal
Imunologia e Infecção
This content is Free Access.
JoVE Journal Imunologia e Infecção
A Semi-automated Approach to Preparing Antibody Cocktails for Immunophenotypic Analysis of Human Peripheral Blood
DOI:

08:17 min

February 08, 2016

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Capítulos

  • 00:05Título
  • 00:58Operating Procedure
  • 06:25Results: Low Variability in Semi-automated Whole Blood Immunophenotyping
  • 07:04Conclusion

Summary

Tadução automática

Whole blood Immunophenotyping is indispensable for monitoring the human immune system. However, the number of reagents required and the instability of specific fluorochromes in premixed cocktails necessitates daily reagent preparation. Here we show that semi-automated preparation of staining antibody cocktail helps establish reliable immunophenotyping by minimizing variability in reagent dispensing.

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