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単離と<em>エクスビボ</emVδ1の>文化<sup> +</sup> CD4<sup> +</sup>γδT細胞、胸腺外αβT細胞前駆
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単離と<em>エクスビボ</emVδ1の>文化<sup> +</sup> CD4<sup> +</sup>γδT細胞、胸腺外αβT細胞前駆
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10:33 min
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December 07, 2015
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Summary
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Here, we provide an optimized protocol for the isolation and cloning of the scarce T-cell entity of peripheral Vδ1+CD4+ T cells that is, as we showed recently, an extrathymic αβ T-cell progenitor. This technique allows to quantitatively isolate, clone and efficiently expand these cells in ex vivo culture.
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Welker, C., Handgretinger, R., Schilbach, K. Isolation and Ex Vivo Culture of Vδ1+CD4+γδ T Cells, an Extrathymic αβT-cell Progenitor. J. Vis. Exp. (106), e53482, doi:10.3791/53482 (2015).
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