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Biologia
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Isolation of Specific Genomic Regions and Identification of Associated Molecules by enChIP
JoVE Journal
Biologia
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JoVE Journal Biologia
Isolation of Specific Genomic Regions and Identification of Associated Molecules by enChIP

Isolation of Specific Genomic Regions and Identification of Associated Molecules by enChIP

DOI:
10.3791/53478-v

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09:26 min

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January 20, 2016

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1Chromatin Biochemistry Research Group, Combined Program on Microbiology and Immunology, Research Institute for Microbial Diseases,Osaka University

Capítulos

  • 00:05Título
  • 00:41Preparation of Tagged Chromatin
  • 04:16Chromatin Immunoprecipitation
  • 06:41Immunoprecipitated Chromatin Analysis
  • 08:08Results: enChIP to Telomere and the Interferon Regulatory Factor-1 (IRF-1) Promoter Regions
  • 09:00Conclusion

Summary

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The identification of molecules associated with specific genomic regions of interest is required to understand the mechanisms of regulation of the functions of these regions. This protocol describes procedures to perform engineered DNA-binding molecule-mediated chromatin imunoprecipitation (enChIP) for identification of proteins and RNAs associated with a specific genomic region.

Tags

Keywords: EnChIPGenomic Region IsolationMolecular InteractionsTranscriptionEpigenetic RegulationDNA-binding MoleculesFLAG-dCas9FLAG-TALCross-linkingFormaldehydeGlycineChromatin ExtractionLysis BufferNuclear Lysis Buffer

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