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Biologia
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جيل وثقافة الدم ثمرة غشائي خلايا من الدم المحيطي الإنسان
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Biologia
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JoVE Journal Biologia
Generation and Culture of Blood Outgrowth Endothelial Cells from Human Peripheral Blood
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جيل وثقافة الدم ثمرة غشائي خلايا من الدم المحيطي الإنسان

18,532 Views

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11:00 min

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December 23, 2015

DOI:

10.3791/53384-v

DOI:
10.3791/53384-v

•

11:00 min
December 23, 2015

•

9 Views
, , , , , ,
1Department of Medicine,University of Cambridge, 2Papworth Hospital

Summary

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This protocol allows for the reliable generation and characterization of blood outgrowth endothelial cells (BOECs) from a small volume of adult peripheral blood. BOECs can be used as a surrogate for endothelial cells from patients with vascular disorders and as a substrate for the generation of induced pluripotent stem cells.

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Blood Outgrowth Endothelial CellsBOECsEndothelial ProgenitorsEndothelial DysfunctionVascular DisordersPrimary Endothelial CellsCell CulturePatient-derivedEndothelial Cell TransplantationInduced Pluripotent Stem CellsIPSCsNuclear ReprogrammingPeripheral Blood

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Cite this Article

Ormiston, M. L., Toshner, M. R., Kiskin, F. N., Huang, C. J. Z., Groves, E., Morrell, N. W., Rana, A. A. Generation and Culture of Blood Outgrowth Endothelial Cells from Human Peripheral Blood. J. Vis. Exp. (106), e53384, doi:10.3791/53384 (2015).

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