Saturable and reverse saturable scattering were discovered in isolated plasmonic particles and adopted as a novel non-bleaching contrast method in super-resolution microscopy. Here the experimental procedures of detecting and extracting nonlinear scattering are explained in detail, as well as how to enhance resolution with the aid of saturated excitation microscopy.
Lee, H., Li, K., Huang, Y., Shen, P., Deka, G., Oketani, R., Yonemaru, Y., Yamanaka, M., Fujita, K., Chu, S. Measurement of Scattering Nonlinearities from a Single Plasmonic Nanoparticle. J. Vis. Exp. (107), e53338, doi:10.3791/53338 (2016).