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自動化されたモジュラーハイスループットエキソポリサッカライドスクリーニングプラットフォームは、高感度炭水化物フィンガープリント分析と相まって
JoVE Journal
Biologia
This content is Free Access.
JoVE Journal Biologia
Automated Modular High Throughput Exopolysaccharide Screening Platform Coupled with Highly Sensitive Carbohydrate Fingerprint Analysis
DOI:

12:02 min

April 11, 2016

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Capítulos

  • 00:05Título
  • 01:04Automated Screening of EPS-producing Strains
  • 04:41Automated Screening: Glucose-assay and Phenol-sulfuric-acid Method
  • 07:00Carbohydrate Fingerprinting: 96-well Micro Hydrolysis
  • 08:41Carbohydrate Fingerprinting: HT-PMP-derivatization
  • 10:06Results: Identification and Characterization of Microbial Exopolysaccharides in High-throughput
  • 11:02Conclusion

Summary

Tadução automática

We present an automated modular high-throughput-method for the identification and characterization of microbial exopolysaccharides in small scale. This method combines a fast preselection to analyze the total amount of secreted polysaccharides with a detailed carbohydrate fingerprint to enable the fast screening of newly isolated bacterial strains or entire strain collections.

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