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फीडर मुक्त परिस्थितियों में सेंडाइ वायरस का उपयोग मानव परिधीय टी कोशिकाओं से प्रेरित स्टेम कोशिकाओं की पीढ़ी
JoVE Journal
Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Generation of Induced Pluripotent Stem Cells from Human Peripheral T Cells Using Sendai Virus in Feeder-free Conditions
DOI:

09:32 min

November 11, 2015

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Capítulos

  • 00:05Título
  • 01:19Preparation of Activated Human T Cells
  • 04:05Generating Induced Pluripotent Stem Cells (iPSCs) from Activated T Cells
  • 07:11Expansion of T Cell-derived iPSCs
  • 08:28Results: Generation of iPSCs from Human Peripheral T Cells
  • 09:03Conclusion

Summary

Tadução automática

This protocol describes how to generate induced pluripotent stem cells (iPSCs) from human peripheral T cells in feeder-free conditions using a combination of matrigel and Sendai virus vectors containing reprogramming factors.

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