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Biologia do Desenvolvimento

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树突棘从锥体神经元的三维定量从人类诱导多能干细胞衍生

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Biologia do Desenvolvimento

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JoVE Journal Biologia do Desenvolvimento

Three-dimensional Quantification of Dendritic Spines from Pyramidal Neurons Derived from Human Induced Pluripotent Stem Cells

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树突棘从锥体神经元的三维定量从人类诱导多能干细胞衍生

DOI:

10.3791/53197-v

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10:18 min

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October 10, 2015

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Laura Gouder^2,3, Jean-Yves Tinevez, Hany Goubran-Botros^2,3, Alexandra Benchoua, Thomas Bourgeron^2,3, Isabelle Cloëz-Tayarani^2,3

¹Human Genetics and Cognitive Functions,Institut Pasteur, ²CNRS URA 2182 ‘Genes, synapses and cognition’,Institut Pasteur, ³Human Genetics and Cognitive Functions,Université Paris Diderot, Sorbonne Paris Cité, ⁴Plateforme d’ Imagerie Dynamique, Imagopole, CiTech,Institut Pasteur, ⁵Neuroplasticity and Therapeutics,CECS, I-STEM, AFM, Evry

Capítulos

00:05Título
01:51Neuronal Culture and Lentiviral Transduction
03:08Immunofluorescence Dendritic Spine Imaging
05:233D Quantification of Dendritic Spines
08:48Results: Spine Quantification of Cultured Dendrites
09:45Conclusion

Summary

Tadução automática

锥体神经元的树突棘是哺乳动物大脑皮质最兴奋性突触的部位。此方法描述在从诱导的多能干细胞衍生的人皮质锥体谷氨酸能神经元脊柱形态的三维定量分析。

Tags

Keywords: Dendritic Spines Pyramidal Neurons Human Induced Pluripotent Stem Cells 3D Quantification Glutamatergic Receptors AMPA NMDA Postsynaptic Densities Neuronal Activity Neuropathological Conditions Neural Stem Cells Cortical Progenitors Psychiatric Diseases

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