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Three-dimensional Quantification of Dendritic Spines from Pyramidal Neurons Derived from Human Induced Pluripotent Stem Cells
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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Three-dimensional Quantification of Dendritic Spines from Pyramidal Neurons Derived from Human Induced Pluripotent Stem Cells

Three-dimensional Quantification of Dendritic Spines from Pyramidal Neurons Derived from Human Induced Pluripotent Stem Cells

DOI:
10.3791/53197-v

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10:18 min

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October 10, 2015

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, , , , ,
1Human Genetics and Cognitive Functions,Institut Pasteur, 2CNRS URA 2182 ‘Genes, synapses and cognition’,Institut Pasteur, 3Human Genetics and Cognitive Functions,Université Paris Diderot, Sorbonne Paris Cité, 4Plateforme d’ Imagerie Dynamique, Imagopole, CiTech,Institut Pasteur, 5Neuroplasticity and Therapeutics,CECS, I-STEM, AFM, Evry

Capítulos

  • 00:05Título
  • 01:51Neuronal Culture and Lentiviral Transduction
  • 03:08Immunofluorescence Dendritic Spine Imaging
  • 05:233D Quantification of Dendritic Spines
  • 08:48Results: Spine Quantification of Cultured Dendrites
  • 09:45Conclusion

Summary

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Dendritic spines of pyramidal neurons are the sites of most excitatory synapses in mammalian brain cortex. This method describes a 3D quantitative analysis of spine morphologies in human cortical pyramidal glutamatergic neurons derived from induced pluripotent stem cells.

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Keywords: Dendritic SpinesPyramidal NeuronsHuman Induced Pluripotent Stem Cells3D QuantificationGlutamatergic ReceptorsAMPANMDAPostsynaptic DensitiesNeuronal ActivityNeuropathological ConditionsNeural Stem CellsCortical ProgenitorsPsychiatric Diseases

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