The technique of diffusive gradients in thin films (DGT) is proposed for speciation studies of plutonium. This protocol describes diffusion experiments probing the behavior of Pu(IV) and Pu(V) in presence of organic matter. DGTs deployed in a karstic spring allow assessment of the bioavailability of Pu.
Cusnir, R., Steinmann, P., Christl, M., Bochud, F., Froidevaux, P. Speciation and Bioavailability Measurements of Environmental Plutonium Using Diffusion in Thin Films. J. Vis. Exp. (105), e53188, doi:10.3791/53188 (2015).