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Die Produktion von Doppelstrang-DNA-Ministrings
JoVE Journal
Biologia
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JoVE Journal Biologia
Production of Double-stranded DNA Ministrings
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Die Produktion von Doppelstrang-DNA-Ministrings

9,988 Views

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06:12 min

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February 29, 2016

DOI:

10.3791/53177-v

DOI:
10.3791/53177-v

•

06:12 min
February 29, 2016

•

2 Views
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1School of Pharmacy,University of Waterloo, 2Sgd Health Innovation

Tags

DNA MinistringsGene DeliveryDNA VectorIn Vivo ProductionOne-step ProductionLinear Covalently Closed DNALB MediumTB MediumAmpicillinW3NN PDMS CellsOvernight CultureMid-log PhaseUV-Vis SpectrophotometryCentrifugationResuspensionInduction

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Wong, S., Lam, P., Nafissi, N., Denniss, S., Slavcev, R. Production of Double-stranded DNA Ministrings. J. Vis. Exp. (108), e53177, doi:10.3791/53177 (2016).

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