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测量TCR-的pMHC结合<em>原位</em>使用基于FRET的显微镜分析
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Bioengenharia
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JoVE Journal Bioengenharia
Measuring TCR-pMHC Binding In Situ using a FRET-based Microscopy Assay
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测量TCR-的pMHC结合<em>原位</em>使用基于FRET的显微镜分析

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19:05 min

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October 30, 2015

DOI:

10.3791/53157-v

DOI:
10.3791/53157-v

•

19:05 min
October 30, 2015

•

6 Views
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1Institute for Applied Physics – Biophysics,Vienna University of Technology, 2Institute for Hygiene and Applied Immunology,Medical University of Vienna

Summary

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This manuscript describes how to conduct (single molecule) Förster Resonance Energy Transfer (FRET)- based assays to measure the binding dynamics between T-cell antigen receptor (TCR) and antigenic peptide-loaded MHC molecules as they occur within the immunological synapse of a T-cell in contact with a functionalized planar supported lipid bilayer.

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Cite this Article

Axmann, M., Schütz, G. J., Huppa, J. B. Measuring TCR-pMHC Binding In Situ using a FRET-based Microscopy Assay. J. Vis. Exp. (104), e53157, doi:10.3791/53157 (2015).

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