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使用非整合附加型质粒从冷冻血沉棕黄层诱导多能干细胞的产生
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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Generation of Induced Pluripotent Stem Cells from Frozen Buffy Coats using Non-integrating Episomal Plasmids
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使用非整合附加型质粒从冷冻血沉棕黄层诱导多能干细胞的产生

DOI:
10.3791/52885-v

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10:52 min

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June 05, 2015

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, , , , , , , , ,
1Center for Biomedicine,European Academy Bozen/Bolzano (EURAC), 2Laboratory of Medical Genetics,Fondazione IRCCS Ca´ Granda, Ospedale Maggiore Policlinico, 3Del E. Webb Center for Neuroscience, Aging & Stem Cell Research,Sanford-Burnham Medical Research Institute

Capítulos

  • 00:05Título
  • 01:44Obtaining Peripheral Blood Mononuclear Cells (PBMNCs) from Buffy Coats
  • 02:46Culturing PBMNCs Obtained from Buffy Coats
  • 04:36Transfecting PBMNCs
  • 06:19Culturing Transfected PBMNCs on Mouse Embryonic Fibroblasts (MEFs)
  • 07:32Generating Induced Pluripotent Stem Cells (iPSC) Clones
  • 08:22Results: Cellular Characteristic from PBMNC to iPSC
  • 10:21Conclusion

Summary

Tadução automática

Tadução automática

诱导多能干细胞（iPS细胞）代表的患者特异性组织为临床应用和基础研究的来源。在这里，我们提出了一个详细的协议来重新编程从冷冻棕黄色大衣将获得使用非整合游离病毒质粒无iPSCs的人外周血单个核细胞（外周血单个核细胞）。

Tags

Keywords: Induced Pluripotent Stem CellsIPSCsNon-integrating Episomal PlasmidsReprogrammingPeripheral Blood Mononuclear CellsPBMNCsFrozen Buffy CoatsRegenerative MedicinePatient-specificTransgene-free

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