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In-vitro- und In-vivo-Modell, um die bakterielle Adhäsion an der Gefäßwand unter Strömungsbedingungen Studieren
JoVE Journal
Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
In Vitro and In Vivo Model to Study Bacterial Adhesion to the Vessel Wall Under Flow Conditions
DOI:

10:24 min

June 11, 2015

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Capítulos

  • 00:05Título
  • 01:40S. aureus Preparation for In Vitro Perfusion and In Vivo Experiments
  • 03:00In Vitro Perfusion Experiments
  • 05:53In Vivo Mesentric Perfusion Model
  • 08:08Results: von Willebrand Factor is Essential for Shear Mediated Adhesion of S. aureus to Endothelial Cells and to the Subendothelial Matrix
  • 09:38Conclusion

Summary

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To study the interaction of bacteria with the blood vessels under shear stress, a flow chamber and an in vivo mesenteric intravital microscopy model are described that allow to dissect the bacterial and host factors contributing to vascular adhesion.

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