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生成和多表型高内涵筛选<em>贝氏柯克斯体</em>转座子突变体

JoVE Journal
Imunologia e Infecção

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JoVE Journal Imunologia e Infecção

Generation and Multi-phenotypic High-content Screening of Coxiella burnetii Transposon Mutants

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生成和多表型高内涵筛选<em>贝氏柯克斯体</em>转座子突变体

DOI:

10.3791/52851-v

•

11:44 min

•

May 13, 2015

•

Eric Martinez, Franck Cantet, Matteo Bonazzi

¹Cell Biology of Bacterial Infections, CNRS, FRE3689, CPBS,Université Montpellier

Capítulos

00:05Título
01:49Isolation of Individual Transposon Mutants
03:44Evaluation of Bacterial Concentration and Single Primer Colony PCR
05:34Eukaryotic Cell Challenge with Coxiella Mutants and Monitoring of Intracellular Growth
07:17Preparation of Samples for Automated Image Acquisition
09:25Results: Viability and Infectivity of Coxiella Transposon Mutants
11:14Conclusion

Summary

Tadução automática

贝氏柯克斯体是专性细胞内革兰阴性细菌负责对人畜共患病Q热。在这里，我们描述了用于Coxiella荧光转座子突变体的产生，以及自动识别和所得内化，复制和细胞毒性表型分析的方法。

Tags

Keywords: Coxiella Burnetii Transposon Mutagenesis GFP-tagged Mutant Library High-content Screening Host-pathogen Interactions Virulence Factors Q Fever Intracellular Bacterial Pathogen

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