Journal
/
/
Metaproteomics זרימת עבודה Aquatic מיקרוביאלית: מתאים לtryptic פפטידים מתאימים לניתוח מבוסס ספקטרומטריית מסת טנדם
JoVE Journal
Ambiente
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Ambiente
An Aquatic Microbial Metaproteomics Workflow: From Cells to Tryptic Peptides Suitable for Tandem Mass Spectrometry-based Analysis
DOI:

08:09 min

September 15, 2015

,

Capítulos

  • 00:05Título
  • 01:06Perform Cell Lysis in Catridge Filter Unit with Cells Preserved in RNA Stabilization Solution
  • 04:07Protein Precipitation and Peptide Extraction
  • 05:25DNA Precipitation
  • 06:49Results: Taxonomic and Functional Analysis of Arctic Ocean Microbial Communities
  • 07:50Conclusion

Summary

Tadução automática

This protocol is for the extraction and concentration of protein and DNA from microbial biomass collected from seawater, followed by the generation of tryptic peptides suitable for tandem mass spectrometry-based proteomic analysis.

Vídeos Relacionados

Read Article