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Un metodo enzimatico per salvare cellule staminali mesenchimali da coagulata midollo osseo da analizzare
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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
An Enzymatic Method to Rescue Mesenchymal Stem Cells from Clotted Bone Marrow Samples
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Un metodo enzimatico per salvare cellule staminali mesenchimali da coagulata midollo osseo da analizzare

9,058 Views

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08:58 min

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April 12, 2015

DOI:

10.3791/52694-v

DOI:
10.3791/52694-v

•

08:58 min
April 12, 2015

•

4 Views
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1Swiss Paraplegic Research, 2Orthopaedics and Spinal Surgery,Swiss Paraplegic Centre, 3Department of Neurosurgery,Lucerne Cantonal Hospital (LUKS), 4Section of Small Animal Surgery/Neurology,Vetsuisse Faculty, University of Zurich

Tags

Mesenchymal Stem CellsBone MarrowUrokinaseClot DigestionCell ExpansionAutologous Stem Cell TherapyHematopoietic SystemClot RemovalEnzymatic DigestionCell QualityChondrogenicOsteogenicAdipogenic Differentiation

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Cite this Article

Schlaefli, P., Bertolo, A., Malonzo, C., Poetzel, T., Baur, M., Steffen, F., Stoyanov, J. An Enzymatic Method to Rescue Mesenchymal Stem Cells from Clotted Bone Marrow Samples. J. Vis. Exp. (98), e52694, doi:10.3791/52694 (2015).

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