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Pairwise צמיחת התחרות Assay לקביעת כושר השכפול של וירוסי כשל חיסוני אנושיים
JoVE Journal
Imunologia e Infecção
This content is Free Access.
JoVE Journal Imunologia e Infecção
Pairwise Growth Competition Assay for Determining the Replication Fitness of Human Immunodeficiency Viruses
DOI:

11:19 min

May 04, 2015

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Capítulos

  • 00:05Título
  • 01:23Growth Competition
  • 05:00Determination of Viral Ratios
  • 09:16Results: HIV Replication Fitness Determined by Pairwise Growth Competition Assays
  • 10:54Conclusion

Summary

Tadução automática

Growth competition between nearly isogenic viruses provides a sensitive measurement for determining relative replication fitness. The protocols described here include the construction of recombinant HIV-1 clones, virus propagation and growth competition and analysis methods optimized to yield sensitive and consistent results.

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