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Immunohistochemie en Multiple Labeling met antilichamen van dezelfde Host te onderzoeken soorten volwassen hippocampus Neurogenese
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Neurociência
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JoVE Journal Neurociência
Immunohistochemistry and Multiple Labeling with Antibodies from the Same Host Species to Study Adult Hippocampal Neurogenesis
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Immunohistochemie en Multiple Labeling met antilichamen van dezelfde Host te onderzoeken soorten volwassen hippocampus Neurogenese

DOI:
10.3791/52551-v

•

09:24 min

•

April 22, 2015

•

, , ,
1Hans Berger Department of Neurology,Jena University Hospital

Capítulos

  • 00:05Título
  • 02:17Injection of Thymidine Analogs and Tissue Preparation
  • 03:38Sequential Multiple-Immunofluorescence with Primary Antibodies from the Same Host Species
  • 06:27Imaging and Data Analysis
  • 07:05Newborn Hippocampal Cells in Cyclin D2 Deficient Mice
  • 08:42Conclusion

Summary

Tadução automática

Tadução automática

This video article illustrates a comprehensive protocol to detect and quantify all stages of adult hippocampal neurogenesis within the same tissue section. We elaborated a method to overcome the limitations of indirect multiple immunofluorescence that arise when suitable antibodies from different host species are unavailable.

Tags

Keywords: Adult NeurogenesisImmunohistochemistryMultiple LabelingAntibodiesHippocampusNeural Stem CellsProgenitor SubtypesProliferation MarkersImmunofluorescenceConfocal MicroscopyCell QuantificationSequential Immunofluorescence

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