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Targeted DNA Methylation Analysis by Next-generation Sequencing
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Biologia
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JoVE Journal Biologia
Targeted DNA Methylation Analysis by Next-generation Sequencing

Targeted DNA Methylation Analysis by Next-generation Sequencing

DOI:
10.3791/52488-v

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08:38 min

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February 24, 2015

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, ,
1Department of Physiology,University of Oklahoma College of Medicine, 2Department of Geriatric Medicine,University of Oklahoma College of Medicine

Capítulos

  • 00:05Título
  • 01:26Bisulfite Specific PCR Optimization
  • 02:33Next Generation Sequencing (NGS) Library Preparation, Library Quality Control, and Methylation Quantification Data Analysis
  • 06:29Results: Representative Methylation Quantification
  • 08:14Conclusion

Summary

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Bisulfite amplicon sequencing (BSAS) is a method for quantifying cytosine methylation in targeted genomic regions of interest. This method uses bisulfite conversion paired with PCR amplification of target regions prior to next-generation sequencing to produce absolute quantitation of DNA methylation at a base-specific level.

Tags

Keywords: DNA MethylationEpigeneticsNext-generation SequencingBisulfite SequencingBisulfite Amplicon SequencingTargeted AnalysisGene ExpressionCytosine MethylationCpG IslandsQuantitative Analysis

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