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Aislamiento, cultivo y mantenimiento a largo plazo de Primaria mesencefálico neuronas dopaminérgicas a partir de cerebros de embriones de roedores
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Neurociência
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JoVE Journal Neurociência
Isolation, Culture and Long-Term Maintenance of Primary Mesencephalic Dopaminergic Neurons From Embryonic Rodent Brains
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Aislamiento, cultivo y mantenimiento a largo plazo de Primaria mesencefálico neuronas dopaminérgicas a partir de cerebros de embriones de roedores

DOI:
10.3791/52475-v

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08:45 min

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February 19, 2015

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, , ,
1Division of Brain Sciences, Department of Medicine,Imperial College London, 2Department of Neurosurgery,Brigham and Women’s Hospital, Harvard Medical School, 3Department of Internal Medicine, Endocrinology,Yale University School of Medicine

Capítulos

  • 00:05Título
  • 01:29Making Preparations
  • 02:45Dissecting the Embryonic Ventral Midbrain
  • 04:44Dissociating the Ventral Midbrain Cells
  • 06:09Plating and Culturing the Ventral Midbrain Cells
  • 07:49Results: Characterizing the Dopaminergic Neuron Cultures
  • 08:21Conclusion

Summary

Tadução automática

Tadução automática

The causes of degeneration of midbrain dopaminergic neurons during Parkinson’s disease are not fully understood. Cellular culture systems provide an essential tool for study of the neurophysiological properties of these neurons. Here we present an optimized protocol, which can be utilized for in vitro modeling of neurodegeneration.

Tags

Keywords: Primary Mesencephalic Dopaminergic NeuronsEmbryonic Rodent BrainsParkinson’s DiseaseNeurodegenerative DisorderCell CultureMidbrain Dopaminergic NeuronsE12.5 MouseE14.5 RatNeuronal MorphologyPhysiological Characteristics

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