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原发性脑多巴胺能神经元的分离，培养和长期维持胚胎脑鼠类
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Neurociência
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JoVE Journal Neurociência
Isolation, Culture and Long-Term Maintenance of Primary Mesencephalic Dopaminergic Neurons From Embryonic Rodent Brains
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原发性脑多巴胺能神经元的分离，培养和长期维持胚胎脑鼠类

13,579 Views

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08:45 min

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February 19, 2015

DOI:

10.3791/52475-v

DOI:
10.3791/52475-v

•

08:45 min
February 19, 2015

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10 Views
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1Division of Brain Sciences, Department of Medicine,Imperial College London, 2Department of Neurosurgery,Brigham and Women’s Hospital, Harvard Medical School, 3Department of Internal Medicine, Endocrinology,Yale University School of Medicine

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Primary Mesencephalic Dopaminergic NeuronsEmbryonic Rodent BrainsParkinson’s DiseaseNeurodegenerative DisorderCell CultureMidbrain Dopaminergic NeuronsE12.5 MouseE14.5 RatNeuronal MorphologyPhysiological Characteristics

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Weinert, M., Selvakumar, T., Tierney, T. S., Alavian, K. N. Isolation, Culture and Long-Term Maintenance of Primary Mesencephalic Dopaminergic Neurons From Embryonic Rodent Brains. J. Vis. Exp. (96), e52475, doi:10.3791/52475 (2015).

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