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Síntesis de indoxil-glucósidos para la detección de glucosidasa Actividades
JoVE Journal
Química
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JoVE Journal Química
Synthesis of Indoxyl-glycosides for Detection of Glycosidase Activities

Síntesis de indoxil-glucósidos para la detección de glucosidasa Actividades

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09:10 min

May 27, 2015

DOI:

09:10 min
May 27, 2015

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Summary

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Indoxyl glycosides are well-established and widely used tools for enzyme screening and enzyme activity monitoring. Especially for glucose type structures previous syntheses proved to be challenging and low yielding. Our novel approach employs indoxylic acid esters as precious intermediates to yield a considerable number of indoxyl glycosides in good yields.

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