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Capítulos
- 00:05Título
- 02:36Fly Dissection and Bacterial Enrichment
- 06:12Perform Lysis for the Detection of Salmonella and Cronobacter
- 07:27PCR Detection of the Target Foodborne Pathogen
- 08:30Isolation of Bacteria from PCR-positive Results
- 10:38Results: Prevalence of Foodborne Pathogens in Wild Flies
- 11:51Conclusion
A PCR-based protocol was adapted to detect Cronobacter spp., Salmonella enterica, and Listeria monocytogenes from body surfaces and alimentary canals of individual wild-caught flies. The goal of this protocol is to detect and isolate bacterial pathogens from individual insects collected as part of an environmental sampling program during foodborne outbreak investigations.
