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对专为递送治疗因素的神经保护策略成人干细胞高通量表征
JoVE Journal
Medicina
This content is Free Access.
JoVE Journal Medicina
High Throughput Characterization of Adult Stem Cells Engineered for Delivery of Therapeutic Factors for Neuroprotective Strategies
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对专为递送治疗因素的神经保护策略成人干细胞高通量表征

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09:19 min

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January 04, 2015

DOI:

10.3791/52242-v

DOI:
10.3791/52242-v

•

09:19 min
January 04, 2015

•

1 Views
, , , , , ,
1Department of Chemical and Biological Engineering,Iowa State University, 2Department of Genetics, Development and Cell Biology,Iowa State University, 3Biology Program,Iowa State University

Summary

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This study describes an experimental platform to rapidly characterize engineered stem cells and their behaviors before their application in long-term in vivo transplant studies for nervous system rescue and repair.

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Mesenchymal Stem CellsGenetic ModificationBrain-derived Neurotrophic FactorGlial Cell-derived Neurotrophic FactorHigh Content ScreeningCell AdhesionCell ProliferationCell MigrationCell ViabilityNeuroprotective Strategies

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Cite this Article

Sharma, A. D., Brodskiy, P. A., Petersen, E. M., Dagdeviren, M., Ye, E., Mallapragada, S. K., Sakaguchi, D. High Throughput Characterization of Adult Stem Cells Engineered for Delivery of Therapeutic Factors for Neuroprotective Strategies. J. Vis. Exp. (95), e52242, doi:10.3791/52242 (2015).

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