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Génération de vecteurs plasmidiques exprimant des protéines de marquage Flag En vertu du règlement de l'allongement Human Factor-1α promoteur Utilisation Assemblée Gibson
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February 09, 2015
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Summary
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Synthesis of custom plasmids is labor and time consuming. This protocol describes the use of Gibson assembly cloning to reduce the work and duration of custom DNA cloning procedure. The protocol described also produces reliable tagged protein constructs for mammalian expression at similar cost to the traditional cut-and-paste DNA cloning.
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Grozdanov, P. N., MacDonald, C. C. Generation of Plasmid Vectors Expressing FLAG-tagged Proteins Under the Regulation of Human Elongation Factor-1α Promoter Using Gibson Assembly. J. Vis. Exp. (96), e52235, doi:10.3791/52235 (2015).
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