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一个程序，植入脊柱商会纵<em>在体内</em>鼠标脊髓成像

JoVE Journal
Neurociência

This content is Free Access.

JoVE Journal Neurociência

A Procedure for Implanting a Spinal Chamber for Longitudinal In Vivo Imaging of the Mouse Spinal Cord

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一个程序，植入脊柱商会纵<em>在体内</em>鼠标脊髓成像

DOI:

10.3791/52196-v

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11:20 min

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December 03, 2014

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Matthew J. Farrar², Chris B. Schaffer

¹Department of Neurobiology and Behavior,Cornell University, ²Department of Biomedical Engineering,Cornell University

Capítulos

00:05Título
01:56Preparing the Mouse for Surgery
02:56Expose the Dorsal Laminae of Thoracic Vertebrae
05:11Perform a Dorsal Laminectomy
06:54Seal the Chamber
09:16Results: Chronic Spinal Chamber Allows Repeated Multi-photon Imaging
10:56Conclusion

Summary

Tadução automática

在这段视频中，我们描述了植入一种慢性的光学成像室在活老鼠的脊髓背侧的过程。室，外科手术，慢性成像的审查和论证。

Tags

Keywords: Spinal Chamber In Vivo Imaging Mouse Spinal Cord Surgical Procedure Optical Access Nonlinear Microscopy Neurodegenerative Insults Chronic Imaging Vertebral Clamping Surgical Implant Post-operative Care Imaging Stability

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