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Régiosélective Biolistic ciblage dans les tranches de cerveau organotypique l'aide d'un pistolet à gènes modifiés
JoVE Journal
Neurociência
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JoVE Journal Neurociência
Regioselective Biolistic Targeting in Organotypic Brain Slices Using a Modified Gene Gun
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Régiosélective Biolistic ciblage dans les tranches de cerveau organotypique l'aide d'un pistolet à gènes modifiés

15,940 Views

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06:40 min

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October 24, 2014

DOI:

10.3791/52148-v

DOI:
10.3791/52148-v

•

06:40 min
October 24, 2014

•

12 Views
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1Leslie Dan Faculty of Pharmacy,University of Toronto, 2MRC-Laboratory of Molecular Biology, Cambridge, UK

Tags

Biolistic TargetingOrganotypic Brain SlicesModified Gene GunDNA TransfectionRegioselective TransfectionTerminally Differentiated NeuronsTransient ExpressionStable ExpressionNanoparticle BombardmentEpisomal ExpressionAnatomical IsolationHeterologous Gene Effects

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Arsenault, J., Nagy, A., Henderson, J. T., O'Brien, J. A. Regioselective Biolistic Targeting in Organotypic Brain Slices Using a Modified Gene Gun. J. Vis. Exp. (92), e52148, doi:10.3791/52148 (2014).

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