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위치 선택성과 유전자는 수정 된 유전자 총을 사용의 Organotypic 뇌 조각에 타겟팅
JoVE Journal
Neurociência
This content is Free Access.
JoVE Journal Neurociência
Regioselective Biolistic Targeting in Organotypic Brain Slices Using a Modified Gene Gun
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위치 선택성과 유전자는 수정 된 유전자 총을 사용의 Organotypic 뇌 조각에 타겟팅

15,975 Views

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06:40 min

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October 24, 2014

DOI:

10.3791/52148-v

DOI:
10.3791/52148-v

•

06:40 min
October 24, 2014

•

6 Views
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1Leslie Dan Faculty of Pharmacy,University of Toronto, 2MRC-Laboratory of Molecular Biology, Cambridge, UK

Summary

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Recent improvements in organotypic brain slice preparations have permitted their exploitation for biotechnological applications. Organotypic slices maintain local structural characteristics of in vivo biology, including functional synaptic connections. Here we present a regioselective biolistic delivery method to label and genetically manipulate these slices.

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Cite this Article

Arsenault, J., Nagy, A., Henderson, J. T., O'Brien, J. A. Regioselective Biolistic Targeting in Organotypic Brain Slices Using a Modified Gene Gun. J. Vis. Exp. (92), e52148, doi:10.3791/52148 (2014).

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