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Associati a cellule intere registrazioni in organotipiche fettine di ippocampo
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Associati a cellule intere registrazioni in organotipiche fettine di ippocampo
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09:23 min
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September 28, 2014
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Pair recordings are simultaneous whole cell patch clamp recordings from two synaptically connected neurons, enabling precise electrophysiological and pharmacological characterization of the synapses between individual neurons. Here we describe the detailed methodology and requirements for establishing this technique in organotypic hippocampal slice cultures in any laboratory equipped for electrophysiology.
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Fourie, C., Kiraly, M., Madison, D. V., Montgomery, J. M. Paired Whole Cell Recordings in Organotypic Hippocampal Slices. J. Vis. Exp. (91), e51958, doi:10.3791/51958 (2014).
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