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Confocal Time Lapse Imaging as an Efficient Method for the Cytocompatibility Evaluation of Dental Composites
JoVE Journal
Medicina
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JoVE Journal Medicina
Confocal Time Lapse Imaging as an Efficient Method for the Cytocompatibility Evaluation of Dental Composites

Confocal Time Lapse Imaging as an Efficient Method for the Cytocompatibility Evaluation of Dental Composites

DOI:
10.3791/51949-v

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07:04 min

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November 09, 2014

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, , ,
1Laboratoire des Multimatériaux et Interfaces,UMR CNRS 5615, Université Lyon1, 2UFR d’Odontologie, Université Lyon1; Service de Consultations et de Traitements Dentaires,Hospices Civils de Lyon, 3UFR d’Odontologie, Université Paris Diderot; Service d’Odontologie,APHP, Hôpital Rothschild

Capítulos

  • 00:05Título
  • 01:25Composite Extract Preparation and Primary Human Gingival Fibroblast (HGF) Cell Culture
  • 02:53Confocal Time Lapse Images
  • 04:38Results: Representative Confocal Time Lapse Images of Composite Extract–composed Cell Cultures
  • 06:16Conclusion

Summary

Tadução automática

Tadução automática

The most studied aspect of the biocompatibility of dental composites is their cytotoxicity 3D CLSM time lapse imaging combined with fluorescent signal quantification allows sensitive evaluation of the cell fate over time. Utilizing this method could be an efficient tool to assess the cytocompatibility of dental composites.

Tags

Confocal Time-lapse ImagingCytocompatibilityDental CompositesELS Extra Low ShrinkagePrimary Human Gingival FibroblastsLive/Dead StainingFV10i Confocal SystemFV10-ASW 3.1 SoftwareIn Vitro Biocompatibility Assessment

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