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כותרות תפוקה גבוהה של וירוסי רקומביננטי לוציפראז- להביע
JoVE Journal
Biologia
This content is Free Access.
JoVE Journal Biologia
High-throughput Titration of Luciferase-expressing Recombinant Viruses
DOI:

08:09 min

September 19, 2014

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Capítulos

  • 00:05Título
  • 01:16Sample Preparation and Preparation of Permissive Cells for Virus Titration
  • 02:57Preparation of Viral Standard Curve and Transfer of Sample Supernatants on to Permissive Cells
  • 04:08Assessment of Cell Viability and Preparation of the Luciferin Substrate
  • 04:53Reading Bioluminescence and Data Analysis
  • 05:46Results: Estimated Viral Titers and Sample Cytotoxicity
  • 07:01Conclusion

Summary

Tadução automática

This article presents a high-throughput luciferase expression-based method of titrating various RNA and DNA viruses using automated and manual liquid handlers.

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