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Fluxo de trabalho para de alta conteúdo, Quantificação de células individuais de marcadores fluorescentes de Microscópio Universal Data, apoiada pela Open Source Software
JoVE Journal
Biologia
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JoVE Journal Biologia
Workflow for High-content, Individual Cell Quantification of Fluorescent Markers from Universal Microscope Data, Supported by Open Source Software

Fluxo de trabalho para de alta conteúdo, Quantificação de células individuais de marcadores fluorescentes de Microscópio Universal Data, apoiada pela Open Source Software

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09:57 min

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December 16, 2014

DOI:

10.3791/51882-v

DOI:
10.3791/51882-v

•

09:57 min
December 16, 2014

•

4 Views
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1Cancer Biology,UCL Cancer Institute

Tags

Cell QuantificationFluorescent MarkersHigh-content MicroscopySingle-cell AnalysisCell HeterogeneityCell ProfilerOpen-source SoftwareSubcellular SegmentationSiRNA ScreenG1 Checkpoint RegulatorsCell PerturbationFluorescence-based Cellular Markers

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Stockwell, S. R., Mittnacht, S. Workflow for High-content, Individual Cell Quantification of Fluorescent Markers from Universal Microscope Data, Supported by Open Source Software. J. Vis. Exp. (94), e51882, doi:10.3791/51882 (2014).

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