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Culturas Organotípicas fatia para Estudar Oligodendrocyte Dynamics e Myelination
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Neurociência
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JoVE Journal Neurociência
Organotypic Slice Cultures to Study Oligodendrocyte Dynamics and Myelination

Culturas Organotípicas fatia para Estudar Oligodendrocyte Dynamics e Myelination

17,989 Views

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09:45 min

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August 25, 2014

DOI:

10.3791/51835-v

DOI:
10.3791/51835-v

•

09:45 min
August 25, 2014

•

9 Views
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1Department of Physiology and Neurobiology,University of Connecticut, 2Stem Cell Institute,University of Connecticut, 3Department of Neurology,Yale University School of Medicine

Summary

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A technique to study NG2 cells and oligodendrocytes using a slice culture system of the forebrain and cerebellum is described. This method allows examination of the dynamics of proliferation and differentiation of cells within the oligodendrocyte lineage where the extracellular environment can be easily manipulated while maintaining tissue cytoarchitecture.

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Hill, R. A., Medved, J., Patel, K. D., Nishiyama, A. Organotypic Slice Cultures to Study Oligodendrocyte Dynamics and Myelination. J. Vis. Exp. (90), e51835, doi:10.3791/51835 (2014).

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