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Directed Dopaminergic Neuron Differentiation from Human Pluripotent Stem Cells
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JoVE Journal Neurociência
Directed Dopaminergic Neuron Differentiation from Human Pluripotent Stem Cells

Directed Dopaminergic Neuron Differentiation from Human Pluripotent Stem Cells

DOI:
10.3791/51737-v

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06:40 min

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September 15, 2014

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, ,
1Institute for Stem Cell Biology and Regenerative Medicine,Stanford University School of Medicine, 2Department of Obstetrics and Gynecology,Stanford University School of Medicine

Capítulos

  • 00:05Título
  • 01:11Preparation of Cells for Differentiation
  • 03:00Cell Differentiation
  • 04:39Results: Representative Dopaminergic Neuron Images
  • 06:13Conclusion

Summary

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We, based on knowledge from developmental biology and published research, developed an optimized protocol to efficiently generate A9 midbrain dopaminergic neurons from both human embryonic stem cells and human induced pluripotent stem cells, which would be useful for disease modeling and cell replacement therapy for Parkinson’s disease.

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Keywords: Dopaminergic NeuronsPluripotent Stem CellsParkinson’s DiseaseCell Replacement TherapyFloor PlateVentral MidlineNeural DevelopmentDifferentiationHuman Embryonic Stem CellsHuman Induced Pluripotent Stem CellsIn Vitro Disease ModelingDrug ScreeningCell Transplantation

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