Journal
/
Biologia
/
Celoppervlaktemerker Mediated Zuivering van iPS Cell Intermediates van een Herprogrammeerbare muismodel
JoVE Journal
Biologia
This content is Free Access.
JoVE Journal Biologia
Cell Surface Marker Mediated Purification of iPS Cell Intermediates from a Reprogrammable Mouse Model
Please note that all translations are automatically generated. Click here for the English version.

Celoppervlaktemerker Mediated Zuivering van iPS Cell Intermediates van een Herprogrammeerbare muismodel

12,073 Views

•

10:32 min

•

September 06, 2014

DOI:

10.3791/51728-v

DOI:
10.3791/51728-v

•

10:32 min
September 06, 2014

•

3 Views
, , , ,
1Department of Anatomy and Developmental Biology,Monash University, 2Australian Regenerative Medicine Institute,Monash University

Summary

Automatically generated

Mouse embryonic fibroblast can be reprogrammed into induced pluripotent stem cells at low efficiency by the forced expression of transcription factors Oct-4, Sox-2, Klf-4, c-Myc. The rare intermediates of the reprogramming reaction are FACS isolated via labeling with antibodies against cell surface makers Thy-1.2, Ssea-1, and Epcam.

Explore More Videos

Cell Surface MarkerIPS CellReprogrammable Mouse ModelInduced Pluripotent Stem CellsReprogrammingCell Surface Marker ExpressionThy-1.2Ssea-1EpcamMouse Embryonic FibroblastsOKSMDoxycyclineFluorescent Activated Cell Sorting

Read Article

Cite this Article

Nefzger, C. M., Alaei, S., Knaupp, A. S., Holmes, M. L., Polo, J. M. Cell Surface Marker Mediated Purification of iPS Cell Intermediates from a Reprogrammable Mouse Model. J. Vis. Exp. (91), e51728, doi:10.3791/51728 (2014).

Download .ris file

Copy

Share Video

.

Copy