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生产单倍体斑马鱼的胚胎通过<em>体外</em>施肥
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July 14, 2014
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The zebrafish is a powerful model system for developmental biology and human disease research due to their genetic similarity with higher vertebrates. This protocol describes a methodology to create haploid zebrafish embryos that can be utilized for forward screen strategies to identify recessive mutations in genes essential for early embryogenesis.
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Kroeger Jr., P. T., Poureetezadi, S. J., McKee, R., Jou, J., Miceli, R., Wingert, R. A. Production of Haploid Zebrafish Embryos by In Vitro Fertilization. J. Vis. Exp. (89), e51708, doi:10.3791/51708 (2014).
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