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人类单核双梯度离心法和其分化为巨噬细胞的特富龙涂层的细胞培养袋隔离
JoVE Journal
Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Isolation of Human Monocytes by Double Gradient Centrifugation and Their Differentiation to Macrophages in Teflon-coated Cell Culture Bags
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人类单核双梯度离心法和其分化为巨噬细胞的特富龙涂层的细胞培养袋隔离

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09:32 min

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September 09, 2014

DOI:

10.3791/51554-v

DOI:
10.3791/51554-v

•

09:32 min
September 09, 2014

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7 Views
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1Department of Hematology and Oncology,University Medical Center Göttingen, 2Microbial Interface Biology Group,Research Center Borstel

Tags

Human MonocytesDouble Gradient CentrifugationMacrophage DifferentiationTeflon-coated Cell Culture BagsM-CSFMacrophage IsolationQuality ControlSyngeneic Human System

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Menck, K., Behme, D., Pantke, M., Reiling, N., Binder, C., Pukrop, T., Klemm, F. Isolation of Human Monocytes by Double Gradient Centrifugation and Their Differentiation to Macrophages in Teflon-coated Cell Culture Bags. J. Vis. Exp. (91), e51554, doi:10.3791/51554 (2014).

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