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Zwei-Photonen-<em> In vivo</em> Imaging von dendritischen Dornen in der Maus Cortex Mit einem ausgedünnten Schädel Vorbereitung
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Zwei-Photonen-<em> In vivo</em> Imaging von dendritischen Dornen in der Maus Cortex Mit einem ausgedünnten Schädel Vorbereitung
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09:53 min
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May 12, 2014
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Summary
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Time-lapse imaging in the living animal provides valuable information on structural reorganization in the intact brain. Here, we introduce a thinned-skull preparation that allows transcranial imaging of fluorescently labeled synaptic structures in the living mouse cortex by two-photon microscopy.
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Yu, X., Zuo, Y. Two-Photon in vivo Imaging of Dendritic Spines in the Mouse Cortex Using a Thinned-skull Preparation. J. Vis. Exp. (87), e51520, doi:10.3791/51520 (2014).
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