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在斑马鱼幼虫脊髓横断

JoVE Journal
Neurociência

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JoVE Journal Neurociência

Spinal Cord Transection in the Larval Zebrafish

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在斑马鱼幼虫脊髓横断

DOI:

10.3791/51479-v

•

06:57 min

•

May 21, 2014

•

Lisa K. Briona, Richard I. Dorsky

¹Department of Neurobiology & Anatomy,University of Utah

Capítulos

00:05Título
01:11Preparation of Surgery Plates and Micropipettes
02:26Preparation of Zebrafish Larvae
03:47Surgery
05:03Recovery
05:55Results: Verifying a Complete Transection
06:38Conclusion

Summary

Tadução automática

脊髓横断后，成年斑马鱼具有功能恢复六个周后，损伤。要利用幼虫的透明度，恢复快的，我们提出了横断幼虫脊髓的方法。横断后，我们通过后3天观察伤感觉恢复在开始后2天伤，和C-弯曲运动。

Tags

Keywords: Spinal Cord Transection Larval Zebrafish Sensory Recovery Motor Recovery Axonal Regrowth Spinal Neurogenesis Regeneration C-bend Movement Free Swimming

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