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Molecular Profiling von der invasiven Tumor-Microenvironment in einem 3-Dimensional Model of Colorectal Cancer Cells und<em> Ex-vivo-</em> Fibroblasten
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JoVE Journal Medicina
Molecular Profiling of the Invasive Tumor Microenvironment in a 3-Dimensional Model of Colorectal Cancer Cells and Ex vivo Fibroblasts
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Molecular Profiling von der invasiven Tumor-Microenvironment in einem 3-Dimensional Model of Colorectal Cancer Cells und<em> Ex-vivo-</em> Fibroblasten

DOI:
10.3791/51475-v

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10:33 min

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April 29, 2014

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, , , , , , , ,
1Cancer Sciences Unit,University of Southampton School of Medicine, 2University Surgical Unit,University of Southampton School of Medicine, 3Bioinformatics Unit,London Research Institute, Cancer Research UK

Capítulos

  • 00:05Título
  • 01:32Establishment of Primary Fibroblast Cultures from Colonic Explants
  • 03:18Organotypic Preparation
  • 06:52Organotypic Fixation and Laser Microdissection of the Invasive Margin
  • 08:47Results: MicroRNA Expression Profiling of Tissue from Laser-microdissected Organotypic CRC Models
  • 10:06Conclusion

Summary

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Molecular Profiling von Laser mikrodissektiert Zellen und Stroma aus synthetischen, Tissue-Engineering-Darmkrebs-Modelle stellt einen neuartigen Ansatz zur manipulierbaren und an der Schnittstelle zwischen Tumorzellen zu charakterisieren und zu sezieren die unverwechselbare Biologie an der invasiven Front und Krebs Stromazellen.

Tags

Molecular ProfilingInvasive Tumor Microenvironment3D Colorectal Cancer ModelEx Vivo FibroblastsTumor InvasionMetastatic CascadeCancer-Associated StromaOrganotypic Co-CultureLaser MicrodissectionGenomic/Transcriptomic/Epigenetic ProfilingBiomarkersDrug Development

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