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Hochdurchsatz-Screening Quantitative Expression und Aufreinigung rekombinanter zu Disulfid-reichen Proteine ​​Venom Produziert in Applied<em> E. coli</em
JoVE Journal
Biologia
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JoVE Journal Biologia
High Throughput Quantitative Expression Screening and Purification Applied to Recombinant Disulfide-rich Venom Proteins Produced in E. coli
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Hochdurchsatz-Screening Quantitative Expression und Aufreinigung rekombinanter zu Disulfid-reichen Proteine ​​Venom Produziert in Applied<em> E. coli</em

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12:16 min

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July 30, 2014

DOI:

10.3791/51464-v

DOI:
10.3791/51464-v

•

12:16 min
July 30, 2014

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5 Views
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1Architecture et Fonction des Macromolécules Biologiques (AFMB),Aix-Marseille Université, 2iBiTec-S, Service d’Ingénierie Moléculaire des Protéines (SIMOPRO),Commissariat à l’énergie atomique et aux énergies alternatives (CEA) Saclay, France

Tags

High ThroughputQuantitative Expression ScreeningRecombinant Disulfide-rich Venom ProteinsE. ColiStructural GenomicsDsbCDisulfide Bond FormationProtein ProductionTherapeutic Drug LeadsVenom ProteinsMass SpectrometryMicro-assays

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Cite this Article

Saez, N. J., Nozach, H., Blemont, M., Vincentelli, R. High Throughput Quantitative Expression Screening and Purification Applied to Recombinant Disulfide-rich Venom Proteins Produced in E. coli. J. Vis. Exp. (89), e51464, doi:10.3791/51464 (2014).

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